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protease iii (acdbio, rnascope multiplex fluorescent reagent kit v2)  (Advanced Cell Diagnostics Inc)


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    Advanced Cell Diagnostics Inc protease iii (acdbio, rnascope multiplex fluorescent reagent kit v2)
    Protease Iii (Acdbio, Rnascope Multiplex Fluorescent Reagent Kit V2), supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope+protease+iii+%26+protease+iv+reagents/protease+iii/10__1096_slash_fj__202500398r-78-13-15
    Average 90 stars, based on 1 article reviews
    protease iii (acdbio, rnascope multiplex fluorescent reagent kit v2) - by Bioz Stars, 2026-09
    90/100 stars

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    Virus:

    Article Title: Skin-type-dependent development of murine mechanosensory neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit polyclonal anti-S100 Beta Fisher 15146-1-AP chicken polyclonal anti-NFH Aves labs NFH0211 rabbit anti-NF200 Sigma N4142 mouse monoclonal anti-NeuN Millipore AB_2298772 rat polyclonal anti-Troma1 DSHB AB_531826 rabbit anti-DsRed Clontech AB_10013483 goat anti-GFP Abcam AB_305635 goat anti-mCherry Cedarlane AB0040-200 guinea pig anti-vGluT1 Millipore AB_2301751 rabbit anti-Homer1 Synaptic Systems 160003 Isolectin B4 647 Invitrogen SCR_014365 Alexa 546 Life Technologies A11056 Alexa 488, 647 Jackson ImmunoResearch 706-605-148, 703-545-155 Bacterial and virus strains AAV2-retro-hSyn-Cre-WPRE Addgene 105553-AAVrg Chemicals, peptides, and recombinant proteins Tamoxifen Sigma T5648-1g BCIP Sigma 11383221001 NBT Sigma 11383213001 Benzyl Alcohol Sigma 402834 Benzyl Benzoate Sigma B-6630 Sunflower seed oil Sigma S5007 Paraformaldehyde, reagent grade, crystalline Millipore Sigma P6148-500G 10% Neutral buffered saline Sigma HT5012-1CS Critical commercial assays SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara 634894 RNAscope Fluorescent Multiplex Assay ACD Bio 320850 RNAscope Protease III & Protease IV Reagents ACD Bio 322340 RNAscope Probe Diluent ACD Bio 300041 RNAscope Fluorescent Multiplex Assay v.2 ACD Bio 323110 RNAscope H2O2 & Protease Reagents ACD Bio 322381 Opal 570 Akoya FP1488001KT Opal 690 Akoya FP1497001KT Opal 520 Akoya FP1487001KT Experimental models: Organisms/strains Mouse: AvilCre Hasegawa et al.39 and JAX 032536 Mouse: Brn3af(AP) Badea et al.25 and JAX 010558 Mouse: Rosa26FSF-LSL-tdTomato (Ai65) JAX 021875 Mouse: RetCreER Luo et al.19 MGI 4437245 Mouse: TrkBCreER Rutlin et al.17 and JAX 027214 Mouse: AdvillinFlp Choi et al.51 N/A Mouse: TrkBGFP Li et al.22 and JAX 023046 Mouse: Npy2rGFP Li et al.22 MGI 3844094 (Continued on next page) e1 Developmental Cell 58, 2032–2047.e1–e6, October 23, 2023

    Article Title: DRG afferents that mediate physiologic and pathologic mechanosensation from the distal colon.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: R26FSF-LSL-tdTomato (Ai65) The Jackson Laboratory Stock No. 021875; RRID:IMSR_JAX:021875 Mouse: R26LSL-tdTomato (Ai14) The Jackson Laboratory Stock No. 007914; RRID:IMSR_JAX:007914 Mouse: R26FSF-tdTomato (derived from Ai65) The Jackson Laboratory Stock No. 021875; RRID:IMSR_JAX:021875 Mouse: R26FRT-LSL-ChR2-YFP-FRT (Ai32) The Jackson Laboratory Stock No. 012569; RRID:IMSR_JAX:012569 Mouse: R26FSF-LSL-ReaChR The Jackson Laboratory Stock No. 024846; RRID:IMSR_JAX:024846 Mouse: R26FRT-LSL-tdTomato-FRT (Ai9) The Jackson Laboratory Stock No. 007909; RRID:IMSR_JAX:007909 Mouse: R26LSL-GCaMP6s (Ai96) The Jackson Laboratory Stock No. 028866; RRID:IMSR_JAX:028866 Mouse: Igs7LSL-GCaMP6f (Ai148) The Jackson Laboratory Stock No. 030328; RRID:IMSR_JAX:030328 Mouse: AdvillinFlp Choi et al.62 N/A Mouse: THCreER The Jackson Laboratory Stock No. 025614; RRID:IMSR_JAX:025614 Mouse: Calca-Flp Choi et al.62 N/A Mouse: Bmpr1bCre Sharma et al.13 N/A Mouse: Sstr2CreER Qi et al.43 N/A Mouse: Smr2Cre Qi et al.43 N/A Mouse: Adra2aCreER Qi et al.43 N/A Mouse: TrkBCreER The Jackson Laboratory Stock No. 027214; MGI:5616440 Mouse: CalcaCreER Song et al.63 N/A Mouse: CalcaLox-GFP-STOP-Lox-hDTR (McCoy et al.36 N/A Mouse: Calca-GFP MMRRC Stock No. 011187-UCD Mouse: Mrgpra3-Cre Han et al.64 N/A Mouse: Mrgprb4CreER Vrontou et al.65 N/A Mouse: PIRTCre Patil et al.47 N/A Oligonucleotides Mm-Piezo2 E43-45 ACD Bio Cat#: 439971 Mm-Bmpr1b ACD Bio Cat#: 533941 Mm-Calca TV2-TV3 ACD Bio Cat#: 420361 Mm-Sstr2 ACD Bio Cat#: 437681 Mm-Adra2a ACD Bio Cat#: 425341 Mm-TrkB (NTRK2) ACD Bio Cat#: 423611 MmTH ACD Bio Cat#: 317621 Mm-Trpv1 ACD Bio Cat#: 313338 Hs-Bmpr1b ACD Bio Cat#: 468031 Hs-Calca ACD Bio Cat#: 605551 GCaMP6s ACD Bio Cat#: 557091 tdTomato ACD Bio Cat#: 317041 Software and algorithms ImageJ/FIJI NIH https://ImageJ.nih.gov/ij/; RRID:SCR_003070 GraphPad-PRISM GraphPad N/A JRCLUST Jun et al.66 https://github.com/ JaneliaSciComp/JRCLUST MATLAB Mathworks https://www.mathworks.com/products/ MATLAB.html; RRID:SCR_001622 Python Van Rossum and Drake67 https://www.python.org/ Bonsai Lopes et al.68 https://bonsai-rx.org/ Other Multielectrode arrays Cambridge Neurotech H4-32ch LED probe ThorLabs 470 nm, 17.2 mW (Min) Fiber-Coupled LED Video camera Fisher Scientific Digital USB 2.0 CMOS Ultrasonic microphone Bat Conservation and Management, Inc. Pettersson M500 microphone (Continued on next page) ll OPEN ACCESS Cell 186, 3368–3385.e1–e8, August 3, 2023 e2 Article

    Article Title: Single-Cell Analysis of Regional Differences in Adult V-SVZ Neural Stem Cell Lineages
    Article Snippet: RNAscope Protease III & Protease IV Reagents , ACDBio , Cat No. 322340.

    Recombinant:

    Article Title: Skin-type-dependent development of murine mechanosensory neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit polyclonal anti-S100 Beta Fisher 15146-1-AP chicken polyclonal anti-NFH Aves labs NFH0211 rabbit anti-NF200 Sigma N4142 mouse monoclonal anti-NeuN Millipore AB_2298772 rat polyclonal anti-Troma1 DSHB AB_531826 rabbit anti-DsRed Clontech AB_10013483 goat anti-GFP Abcam AB_305635 goat anti-mCherry Cedarlane AB0040-200 guinea pig anti-vGluT1 Millipore AB_2301751 rabbit anti-Homer1 Synaptic Systems 160003 Isolectin B4 647 Invitrogen SCR_014365 Alexa 546 Life Technologies A11056 Alexa 488, 647 Jackson ImmunoResearch 706-605-148, 703-545-155 Bacterial and virus strains AAV2-retro-hSyn-Cre-WPRE Addgene 105553-AAVrg Chemicals, peptides, and recombinant proteins Tamoxifen Sigma T5648-1g BCIP Sigma 11383221001 NBT Sigma 11383213001 Benzyl Alcohol Sigma 402834 Benzyl Benzoate Sigma B-6630 Sunflower seed oil Sigma S5007 Paraformaldehyde, reagent grade, crystalline Millipore Sigma P6148-500G 10% Neutral buffered saline Sigma HT5012-1CS Critical commercial assays SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara 634894 RNAscope Fluorescent Multiplex Assay ACD Bio 320850 RNAscope Protease III & Protease IV Reagents ACD Bio 322340 RNAscope Probe Diluent ACD Bio 300041 RNAscope Fluorescent Multiplex Assay v.2 ACD Bio 323110 RNAscope H2O2 & Protease Reagents ACD Bio 322381 Opal 570 Akoya FP1488001KT Opal 690 Akoya FP1497001KT Opal 520 Akoya FP1487001KT Experimental models: Organisms/strains Mouse: AvilCre Hasegawa et al.39 and JAX 032536 Mouse: Brn3af(AP) Badea et al.25 and JAX 010558 Mouse: Rosa26FSF-LSL-tdTomato (Ai65) JAX 021875 Mouse: RetCreER Luo et al.19 MGI 4437245 Mouse: TrkBCreER Rutlin et al.17 and JAX 027214 Mouse: AdvillinFlp Choi et al.51 N/A Mouse: TrkBGFP Li et al.22 and JAX 023046 Mouse: Npy2rGFP Li et al.22 MGI 3844094 (Continued on next page) e1 Developmental Cell 58, 2032–2047.e1–e6, October 23, 2023

    Article Title: DRG afferents that mediate physiologic and pathologic mechanosensation from the distal colon.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: R26FSF-LSL-tdTomato (Ai65) The Jackson Laboratory Stock No. 021875; RRID:IMSR_JAX:021875 Mouse: R26LSL-tdTomato (Ai14) The Jackson Laboratory Stock No. 007914; RRID:IMSR_JAX:007914 Mouse: R26FSF-tdTomato (derived from Ai65) The Jackson Laboratory Stock No. 021875; RRID:IMSR_JAX:021875 Mouse: R26FRT-LSL-ChR2-YFP-FRT (Ai32) The Jackson Laboratory Stock No. 012569; RRID:IMSR_JAX:012569 Mouse: R26FSF-LSL-ReaChR The Jackson Laboratory Stock No. 024846; RRID:IMSR_JAX:024846 Mouse: R26FRT-LSL-tdTomato-FRT (Ai9) The Jackson Laboratory Stock No. 007909; RRID:IMSR_JAX:007909 Mouse: R26LSL-GCaMP6s (Ai96) The Jackson Laboratory Stock No. 028866; RRID:IMSR_JAX:028866 Mouse: Igs7LSL-GCaMP6f (Ai148) The Jackson Laboratory Stock No. 030328; RRID:IMSR_JAX:030328 Mouse: AdvillinFlp Choi et al.62 N/A Mouse: THCreER The Jackson Laboratory Stock No. 025614; RRID:IMSR_JAX:025614 Mouse: Calca-Flp Choi et al.62 N/A Mouse: Bmpr1bCre Sharma et al.13 N/A Mouse: Sstr2CreER Qi et al.43 N/A Mouse: Smr2Cre Qi et al.43 N/A Mouse: Adra2aCreER Qi et al.43 N/A Mouse: TrkBCreER The Jackson Laboratory Stock No. 027214; MGI:5616440 Mouse: CalcaCreER Song et al.63 N/A Mouse: CalcaLox-GFP-STOP-Lox-hDTR (McCoy et al.36 N/A Mouse: Calca-GFP MMRRC Stock No. 011187-UCD Mouse: Mrgpra3-Cre Han et al.64 N/A Mouse: Mrgprb4CreER Vrontou et al.65 N/A Mouse: PIRTCre Patil et al.47 N/A Oligonucleotides Mm-Piezo2 E43-45 ACD Bio Cat#: 439971 Mm-Bmpr1b ACD Bio Cat#: 533941 Mm-Calca TV2-TV3 ACD Bio Cat#: 420361 Mm-Sstr2 ACD Bio Cat#: 437681 Mm-Adra2a ACD Bio Cat#: 425341 Mm-TrkB (NTRK2) ACD Bio Cat#: 423611 MmTH ACD Bio Cat#: 317621 Mm-Trpv1 ACD Bio Cat#: 313338 Hs-Bmpr1b ACD Bio Cat#: 468031 Hs-Calca ACD Bio Cat#: 605551 GCaMP6s ACD Bio Cat#: 557091 tdTomato ACD Bio Cat#: 317041 Software and algorithms ImageJ/FIJI NIH https://ImageJ.nih.gov/ij/; RRID:SCR_003070 GraphPad-PRISM GraphPad N/A JRCLUST Jun et al.66 https://github.com/ JaneliaSciComp/JRCLUST MATLAB Mathworks https://www.mathworks.com/products/ MATLAB.html; RRID:SCR_001622 Python Van Rossum and Drake67 https://www.python.org/ Bonsai Lopes et al.68 https://bonsai-rx.org/ Other Multielectrode arrays Cambridge Neurotech H4-32ch LED probe ThorLabs 470 nm, 17.2 mW (Min) Fiber-Coupled LED Video camera Fisher Scientific Digital USB 2.0 CMOS Ultrasonic microphone Bat Conservation and Management, Inc. Pettersson M500 microphone (Continued on next page) ll OPEN ACCESS Cell 186, 3368–3385.e1–e8, August 3, 2023 e2 Article

    Article Title: Single-Cell Analysis of Regional Differences in Adult V-SVZ Neural Stem Cell Lineages
    Article Snippet: RNAscope Protease III & Protease IV Reagents , ACDBio , Cat No. 322340.

    Saline:

    Article Title: Skin-type-dependent development of murine mechanosensory neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit polyclonal anti-S100 Beta Fisher 15146-1-AP chicken polyclonal anti-NFH Aves labs NFH0211 rabbit anti-NF200 Sigma N4142 mouse monoclonal anti-NeuN Millipore AB_2298772 rat polyclonal anti-Troma1 DSHB AB_531826 rabbit anti-DsRed Clontech AB_10013483 goat anti-GFP Abcam AB_305635 goat anti-mCherry Cedarlane AB0040-200 guinea pig anti-vGluT1 Millipore AB_2301751 rabbit anti-Homer1 Synaptic Systems 160003 Isolectin B4 647 Invitrogen SCR_014365 Alexa 546 Life Technologies A11056 Alexa 488, 647 Jackson ImmunoResearch 706-605-148, 703-545-155 Bacterial and virus strains AAV2-retro-hSyn-Cre-WPRE Addgene 105553-AAVrg Chemicals, peptides, and recombinant proteins Tamoxifen Sigma T5648-1g BCIP Sigma 11383221001 NBT Sigma 11383213001 Benzyl Alcohol Sigma 402834 Benzyl Benzoate Sigma B-6630 Sunflower seed oil Sigma S5007 Paraformaldehyde, reagent grade, crystalline Millipore Sigma P6148-500G 10% Neutral buffered saline Sigma HT5012-1CS Critical commercial assays SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara 634894 RNAscope Fluorescent Multiplex Assay ACD Bio 320850 RNAscope Protease III & Protease IV Reagents ACD Bio 322340 RNAscope Probe Diluent ACD Bio 300041 RNAscope Fluorescent Multiplex Assay v.2 ACD Bio 323110 RNAscope H2O2 & Protease Reagents ACD Bio 322381 Opal 570 Akoya FP1488001KT Opal 690 Akoya FP1497001KT Opal 520 Akoya FP1487001KT Experimental models: Organisms/strains Mouse: AvilCre Hasegawa et al.39 and JAX 032536 Mouse: Brn3af(AP) Badea et al.25 and JAX 010558 Mouse: Rosa26FSF-LSL-tdTomato (Ai65) JAX 021875 Mouse: RetCreER Luo et al.19 MGI 4437245 Mouse: TrkBCreER Rutlin et al.17 and JAX 027214 Mouse: AdvillinFlp Choi et al.51 N/A Mouse: TrkBGFP Li et al.22 and JAX 023046 Mouse: Npy2rGFP Li et al.22 MGI 3844094 (Continued on next page) e1 Developmental Cell 58, 2032–2047.e1–e6, October 23, 2023

    Article Title: DRG afferents that mediate physiologic and pathologic mechanosensation from the distal colon.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: R26FSF-LSL-tdTomato (Ai65) The Jackson Laboratory Stock No. 021875; RRID:IMSR_JAX:021875 Mouse: R26LSL-tdTomato (Ai14) The Jackson Laboratory Stock No. 007914; RRID:IMSR_JAX:007914 Mouse: R26FSF-tdTomato (derived from Ai65) The Jackson Laboratory Stock No. 021875; RRID:IMSR_JAX:021875 Mouse: R26FRT-LSL-ChR2-YFP-FRT (Ai32) The Jackson Laboratory Stock No. 012569; RRID:IMSR_JAX:012569 Mouse: R26FSF-LSL-ReaChR The Jackson Laboratory Stock No. 024846; RRID:IMSR_JAX:024846 Mouse: R26FRT-LSL-tdTomato-FRT (Ai9) The Jackson Laboratory Stock No. 007909; RRID:IMSR_JAX:007909 Mouse: R26LSL-GCaMP6s (Ai96) The Jackson Laboratory Stock No. 028866; RRID:IMSR_JAX:028866 Mouse: Igs7LSL-GCaMP6f (Ai148) The Jackson Laboratory Stock No. 030328; RRID:IMSR_JAX:030328 Mouse: AdvillinFlp Choi et al.62 N/A Mouse: THCreER The Jackson Laboratory Stock No. 025614; RRID:IMSR_JAX:025614 Mouse: Calca-Flp Choi et al.62 N/A Mouse: Bmpr1bCre Sharma et al.13 N/A Mouse: Sstr2CreER Qi et al.43 N/A Mouse: Smr2Cre Qi et al.43 N/A Mouse: Adra2aCreER Qi et al.43 N/A Mouse: TrkBCreER The Jackson Laboratory Stock No. 027214; MGI:5616440 Mouse: CalcaCreER Song et al.63 N/A Mouse: CalcaLox-GFP-STOP-Lox-hDTR (McCoy et al.36 N/A Mouse: Calca-GFP MMRRC Stock No. 011187-UCD Mouse: Mrgpra3-Cre Han et al.64 N/A Mouse: Mrgprb4CreER Vrontou et al.65 N/A Mouse: PIRTCre Patil et al.47 N/A Oligonucleotides Mm-Piezo2 E43-45 ACD Bio Cat#: 439971 Mm-Bmpr1b ACD Bio Cat#: 533941 Mm-Calca TV2-TV3 ACD Bio Cat#: 420361 Mm-Sstr2 ACD Bio Cat#: 437681 Mm-Adra2a ACD Bio Cat#: 425341 Mm-TrkB (NTRK2) ACD Bio Cat#: 423611 MmTH ACD Bio Cat#: 317621 Mm-Trpv1 ACD Bio Cat#: 313338 Hs-Bmpr1b ACD Bio Cat#: 468031 Hs-Calca ACD Bio Cat#: 605551 GCaMP6s ACD Bio Cat#: 557091 tdTomato ACD Bio Cat#: 317041 Software and algorithms ImageJ/FIJI NIH https://ImageJ.nih.gov/ij/; RRID:SCR_003070 GraphPad-PRISM GraphPad N/A JRCLUST Jun et al.66 https://github.com/ JaneliaSciComp/JRCLUST MATLAB Mathworks https://www.mathworks.com/products/ MATLAB.html; RRID:SCR_001622 Python Van Rossum and Drake67 https://www.python.org/ Bonsai Lopes et al.68 https://bonsai-rx.org/ Other Multielectrode arrays Cambridge Neurotech H4-32ch LED probe ThorLabs 470 nm, 17.2 mW (Min) Fiber-Coupled LED Video camera Fisher Scientific Digital USB 2.0 CMOS Ultrasonic microphone Bat Conservation and Management, Inc. Pettersson M500 microphone (Continued on next page) ll OPEN ACCESS Cell 186, 3368–3385.e1–e8, August 3, 2023 e2 Article

    Article Title: Single-Cell Analysis of Regional Differences in Adult V-SVZ Neural Stem Cell Lineages
    Article Snippet: RNAscope Protease III & Protease IV Reagents , ACDBio , Cat No. 322340.

    Sequencing:

    Article Title: Skin-type-dependent development of murine mechanosensory neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit polyclonal anti-S100 Beta Fisher 15146-1-AP chicken polyclonal anti-NFH Aves labs NFH0211 rabbit anti-NF200 Sigma N4142 mouse monoclonal anti-NeuN Millipore AB_2298772 rat polyclonal anti-Troma1 DSHB AB_531826 rabbit anti-DsRed Clontech AB_10013483 goat anti-GFP Abcam AB_305635 goat anti-mCherry Cedarlane AB0040-200 guinea pig anti-vGluT1 Millipore AB_2301751 rabbit anti-Homer1 Synaptic Systems 160003 Isolectin B4 647 Invitrogen SCR_014365 Alexa 546 Life Technologies A11056 Alexa 488, 647 Jackson ImmunoResearch 706-605-148, 703-545-155 Bacterial and virus strains AAV2-retro-hSyn-Cre-WPRE Addgene 105553-AAVrg Chemicals, peptides, and recombinant proteins Tamoxifen Sigma T5648-1g BCIP Sigma 11383221001 NBT Sigma 11383213001 Benzyl Alcohol Sigma 402834 Benzyl Benzoate Sigma B-6630 Sunflower seed oil Sigma S5007 Paraformaldehyde, reagent grade, crystalline Millipore Sigma P6148-500G 10% Neutral buffered saline Sigma HT5012-1CS Critical commercial assays SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara 634894 RNAscope Fluorescent Multiplex Assay ACD Bio 320850 RNAscope Protease III & Protease IV Reagents ACD Bio 322340 RNAscope Probe Diluent ACD Bio 300041 RNAscope Fluorescent Multiplex Assay v.2 ACD Bio 323110 RNAscope H2O2 & Protease Reagents ACD Bio 322381 Opal 570 Akoya FP1488001KT Opal 690 Akoya FP1497001KT Opal 520 Akoya FP1487001KT Experimental models: Organisms/strains Mouse: AvilCre Hasegawa et al.39 and JAX 032536 Mouse: Brn3af(AP) Badea et al.25 and JAX 010558 Mouse: Rosa26FSF-LSL-tdTomato (Ai65) JAX 021875 Mouse: RetCreER Luo et al.19 MGI 4437245 Mouse: TrkBCreER Rutlin et al.17 and JAX 027214 Mouse: AdvillinFlp Choi et al.51 N/A Mouse: TrkBGFP Li et al.22 and JAX 023046 Mouse: Npy2rGFP Li et al.22 MGI 3844094 (Continued on next page) e1 Developmental Cell 58, 2032–2047.e1–e6, October 23, 2023

    Article Title: DRG afferents that mediate physiologic and pathologic mechanosensation from the distal colon.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: R26FSF-LSL-tdTomato (Ai65) The Jackson Laboratory Stock No. 021875; RRID:IMSR_JAX:021875 Mouse: R26LSL-tdTomato (Ai14) The Jackson Laboratory Stock No. 007914; RRID:IMSR_JAX:007914 Mouse: R26FSF-tdTomato (derived from Ai65) The Jackson Laboratory Stock No. 021875; RRID:IMSR_JAX:021875 Mouse: R26FRT-LSL-ChR2-YFP-FRT (Ai32) The Jackson Laboratory Stock No. 012569; RRID:IMSR_JAX:012569 Mouse: R26FSF-LSL-ReaChR The Jackson Laboratory Stock No. 024846; RRID:IMSR_JAX:024846 Mouse: R26FRT-LSL-tdTomato-FRT (Ai9) The Jackson Laboratory Stock No. 007909; RRID:IMSR_JAX:007909 Mouse: R26LSL-GCaMP6s (Ai96) The Jackson Laboratory Stock No. 028866; RRID:IMSR_JAX:028866 Mouse: Igs7LSL-GCaMP6f (Ai148) The Jackson Laboratory Stock No. 030328; RRID:IMSR_JAX:030328 Mouse: AdvillinFlp Choi et al.62 N/A Mouse: THCreER The Jackson Laboratory Stock No. 025614; RRID:IMSR_JAX:025614 Mouse: Calca-Flp Choi et al.62 N/A Mouse: Bmpr1bCre Sharma et al.13 N/A Mouse: Sstr2CreER Qi et al.43 N/A Mouse: Smr2Cre Qi et al.43 N/A Mouse: Adra2aCreER Qi et al.43 N/A Mouse: TrkBCreER The Jackson Laboratory Stock No. 027214; MGI:5616440 Mouse: CalcaCreER Song et al.63 N/A Mouse: CalcaLox-GFP-STOP-Lox-hDTR (McCoy et al.36 N/A Mouse: Calca-GFP MMRRC Stock No. 011187-UCD Mouse: Mrgpra3-Cre Han et al.64 N/A Mouse: Mrgprb4CreER Vrontou et al.65 N/A Mouse: PIRTCre Patil et al.47 N/A Oligonucleotides Mm-Piezo2 E43-45 ACD Bio Cat#: 439971 Mm-Bmpr1b ACD Bio Cat#: 533941 Mm-Calca TV2-TV3 ACD Bio Cat#: 420361 Mm-Sstr2 ACD Bio Cat#: 437681 Mm-Adra2a ACD Bio Cat#: 425341 Mm-TrkB (NTRK2) ACD Bio Cat#: 423611 MmTH ACD Bio Cat#: 317621 Mm-Trpv1 ACD Bio Cat#: 313338 Hs-Bmpr1b ACD Bio Cat#: 468031 Hs-Calca ACD Bio Cat#: 605551 GCaMP6s ACD Bio Cat#: 557091 tdTomato ACD Bio Cat#: 317041 Software and algorithms ImageJ/FIJI NIH https://ImageJ.nih.gov/ij/; RRID:SCR_003070 GraphPad-PRISM GraphPad N/A JRCLUST Jun et al.66 https://github.com/ JaneliaSciComp/JRCLUST MATLAB Mathworks https://www.mathworks.com/products/ MATLAB.html; RRID:SCR_001622 Python Van Rossum and Drake67 https://www.python.org/ Bonsai Lopes et al.68 https://bonsai-rx.org/ Other Multielectrode arrays Cambridge Neurotech H4-32ch LED probe ThorLabs 470 nm, 17.2 mW (Min) Fiber-Coupled LED Video camera Fisher Scientific Digital USB 2.0 CMOS Ultrasonic microphone Bat Conservation and Management, Inc. Pettersson M500 microphone (Continued on next page) ll OPEN ACCESS Cell 186, 3368–3385.e1–e8, August 3, 2023 e2 Article

    Article Title: Single-Cell Analysis of Regional Differences in Adult V-SVZ Neural Stem Cell Lineages
    Article Snippet: RNAscope Protease III & Protease IV Reagents , ACDBio , Cat No. 322340.

    RNAscope:

    Article Title: Skin-type-dependent development of murine mechanosensory neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit polyclonal anti-S100 Beta Fisher 15146-1-AP chicken polyclonal anti-NFH Aves labs NFH0211 rabbit anti-NF200 Sigma N4142 mouse monoclonal anti-NeuN Millipore AB_2298772 rat polyclonal anti-Troma1 DSHB AB_531826 rabbit anti-DsRed Clontech AB_10013483 goat anti-GFP Abcam AB_305635 goat anti-mCherry Cedarlane AB0040-200 guinea pig anti-vGluT1 Millipore AB_2301751 rabbit anti-Homer1 Synaptic Systems 160003 Isolectin B4 647 Invitrogen SCR_014365 Alexa 546 Life Technologies A11056 Alexa 488, 647 Jackson ImmunoResearch 706-605-148, 703-545-155 Bacterial and virus strains AAV2-retro-hSyn-Cre-WPRE Addgene 105553-AAVrg Chemicals, peptides, and recombinant proteins Tamoxifen Sigma T5648-1g BCIP Sigma 11383221001 NBT Sigma 11383213001 Benzyl Alcohol Sigma 402834 Benzyl Benzoate Sigma B-6630 Sunflower seed oil Sigma S5007 Paraformaldehyde, reagent grade, crystalline Millipore Sigma P6148-500G 10% Neutral buffered saline Sigma HT5012-1CS Critical commercial assays SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara 634894 RNAscope Fluorescent Multiplex Assay ACD Bio 320850 RNAscope Protease III & Protease IV Reagents ACD Bio 322340 RNAscope Probe Diluent ACD Bio 300041 RNAscope Fluorescent Multiplex Assay v.2 ACD Bio 323110 RNAscope H2O2 & Protease Reagents ACD Bio 322381 Opal 570 Akoya FP1488001KT Opal 690 Akoya FP1497001KT Opal 520 Akoya FP1487001KT Experimental models: Organisms/strains Mouse: AvilCre Hasegawa et al.39 and JAX 032536 Mouse: Brn3af(AP) Badea et al.25 and JAX 010558 Mouse: Rosa26FSF-LSL-tdTomato (Ai65) JAX 021875 Mouse: RetCreER Luo et al.19 MGI 4437245 Mouse: TrkBCreER Rutlin et al.17 and JAX 027214 Mouse: AdvillinFlp Choi et al.51 N/A Mouse: TrkBGFP Li et al.22 and JAX 023046 Mouse: Npy2rGFP Li et al.22 MGI 3844094 (Continued on next page) e1 Developmental Cell 58, 2032–2047.e1–e6, October 23, 2023

    Article Title: DRG afferents that mediate physiologic and pathologic mechanosensation from the distal colon.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: R26FSF-LSL-tdTomato (Ai65) The Jackson Laboratory Stock No. 021875; RRID:IMSR_JAX:021875 Mouse: R26LSL-tdTomato (Ai14) The Jackson Laboratory Stock No. 007914; RRID:IMSR_JAX:007914 Mouse: R26FSF-tdTomato (derived from Ai65) The Jackson Laboratory Stock No. 021875; RRID:IMSR_JAX:021875 Mouse: R26FRT-LSL-ChR2-YFP-FRT (Ai32) The Jackson Laboratory Stock No. 012569; RRID:IMSR_JAX:012569 Mouse: R26FSF-LSL-ReaChR The Jackson Laboratory Stock No. 024846; RRID:IMSR_JAX:024846 Mouse: R26FRT-LSL-tdTomato-FRT (Ai9) The Jackson Laboratory Stock No. 007909; RRID:IMSR_JAX:007909 Mouse: R26LSL-GCaMP6s (Ai96) The Jackson Laboratory Stock No. 028866; RRID:IMSR_JAX:028866 Mouse: Igs7LSL-GCaMP6f (Ai148) The Jackson Laboratory Stock No. 030328; RRID:IMSR_JAX:030328 Mouse: AdvillinFlp Choi et al.62 N/A Mouse: THCreER The Jackson Laboratory Stock No. 025614; RRID:IMSR_JAX:025614 Mouse: Calca-Flp Choi et al.62 N/A Mouse: Bmpr1bCre Sharma et al.13 N/A Mouse: Sstr2CreER Qi et al.43 N/A Mouse: Smr2Cre Qi et al.43 N/A Mouse: Adra2aCreER Qi et al.43 N/A Mouse: TrkBCreER The Jackson Laboratory Stock No. 027214; MGI:5616440 Mouse: CalcaCreER Song et al.63 N/A Mouse: CalcaLox-GFP-STOP-Lox-hDTR (McCoy et al.36 N/A Mouse: Calca-GFP MMRRC Stock No. 011187-UCD Mouse: Mrgpra3-Cre Han et al.64 N/A Mouse: Mrgprb4CreER Vrontou et al.65 N/A Mouse: PIRTCre Patil et al.47 N/A Oligonucleotides Mm-Piezo2 E43-45 ACD Bio Cat#: 439971 Mm-Bmpr1b ACD Bio Cat#: 533941 Mm-Calca TV2-TV3 ACD Bio Cat#: 420361 Mm-Sstr2 ACD Bio Cat#: 437681 Mm-Adra2a ACD Bio Cat#: 425341 Mm-TrkB (NTRK2) ACD Bio Cat#: 423611 MmTH ACD Bio Cat#: 317621 Mm-Trpv1 ACD Bio Cat#: 313338 Hs-Bmpr1b ACD Bio Cat#: 468031 Hs-Calca ACD Bio Cat#: 605551 GCaMP6s ACD Bio Cat#: 557091 tdTomato ACD Bio Cat#: 317041 Software and algorithms ImageJ/FIJI NIH https://ImageJ.nih.gov/ij/; RRID:SCR_003070 GraphPad-PRISM GraphPad N/A JRCLUST Jun et al.66 https://github.com/ JaneliaSciComp/JRCLUST MATLAB Mathworks https://www.mathworks.com/products/ MATLAB.html; RRID:SCR_001622 Python Van Rossum and Drake67 https://www.python.org/ Bonsai Lopes et al.68 https://bonsai-rx.org/ Other Multielectrode arrays Cambridge Neurotech H4-32ch LED probe ThorLabs 470 nm, 17.2 mW (Min) Fiber-Coupled LED Video camera Fisher Scientific Digital USB 2.0 CMOS Ultrasonic microphone Bat Conservation and Management, Inc. Pettersson M500 microphone (Continued on next page) ll OPEN ACCESS Cell 186, 3368–3385.e1–e8, August 3, 2023 e2 Article

    Article Title: Single-Cell Analysis of Regional Differences in Adult V-SVZ Neural Stem Cell Lineages
    Article Snippet: RNAscope Protease III & Protease IV Reagents , ACDBio , Cat No. 322340.

    Multiplex Assay:

    Article Title: Skin-type-dependent development of murine mechanosensory neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit polyclonal anti-S100 Beta Fisher 15146-1-AP chicken polyclonal anti-NFH Aves labs NFH0211 rabbit anti-NF200 Sigma N4142 mouse monoclonal anti-NeuN Millipore AB_2298772 rat polyclonal anti-Troma1 DSHB AB_531826 rabbit anti-DsRed Clontech AB_10013483 goat anti-GFP Abcam AB_305635 goat anti-mCherry Cedarlane AB0040-200 guinea pig anti-vGluT1 Millipore AB_2301751 rabbit anti-Homer1 Synaptic Systems 160003 Isolectin B4 647 Invitrogen SCR_014365 Alexa 546 Life Technologies A11056 Alexa 488, 647 Jackson ImmunoResearch 706-605-148, 703-545-155 Bacterial and virus strains AAV2-retro-hSyn-Cre-WPRE Addgene 105553-AAVrg Chemicals, peptides, and recombinant proteins Tamoxifen Sigma T5648-1g BCIP Sigma 11383221001 NBT Sigma 11383213001 Benzyl Alcohol Sigma 402834 Benzyl Benzoate Sigma B-6630 Sunflower seed oil Sigma S5007 Paraformaldehyde, reagent grade, crystalline Millipore Sigma P6148-500G 10% Neutral buffered saline Sigma HT5012-1CS Critical commercial assays SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara 634894 RNAscope Fluorescent Multiplex Assay ACD Bio 320850 RNAscope Protease III & Protease IV Reagents ACD Bio 322340 RNAscope Probe Diluent ACD Bio 300041 RNAscope Fluorescent Multiplex Assay v.2 ACD Bio 323110 RNAscope H2O2 & Protease Reagents ACD Bio 322381 Opal 570 Akoya FP1488001KT Opal 690 Akoya FP1497001KT Opal 520 Akoya FP1487001KT Experimental models: Organisms/strains Mouse: AvilCre Hasegawa et al.39 and JAX 032536 Mouse: Brn3af(AP) Badea et al.25 and JAX 010558 Mouse: Rosa26FSF-LSL-tdTomato (Ai65) JAX 021875 Mouse: RetCreER Luo et al.19 MGI 4437245 Mouse: TrkBCreER Rutlin et al.17 and JAX 027214 Mouse: AdvillinFlp Choi et al.51 N/A Mouse: TrkBGFP Li et al.22 and JAX 023046 Mouse: Npy2rGFP Li et al.22 MGI 3844094 (Continued on next page) e1 Developmental Cell 58, 2032–2047.e1–e6, October 23, 2023

    Article Title: DRG afferents that mediate physiologic and pathologic mechanosensation from the distal colon.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: R26FSF-LSL-tdTomato (Ai65) The Jackson Laboratory Stock No. 021875; RRID:IMSR_JAX:021875 Mouse: R26LSL-tdTomato (Ai14) The Jackson Laboratory Stock No. 007914; RRID:IMSR_JAX:007914 Mouse: R26FSF-tdTomato (derived from Ai65) The Jackson Laboratory Stock No. 021875; RRID:IMSR_JAX:021875 Mouse: R26FRT-LSL-ChR2-YFP-FRT (Ai32) The Jackson Laboratory Stock No. 012569; RRID:IMSR_JAX:012569 Mouse: R26FSF-LSL-ReaChR The Jackson Laboratory Stock No. 024846; RRID:IMSR_JAX:024846 Mouse: R26FRT-LSL-tdTomato-FRT (Ai9) The Jackson Laboratory Stock No. 007909; RRID:IMSR_JAX:007909 Mouse: R26LSL-GCaMP6s (Ai96) The Jackson Laboratory Stock No. 028866; RRID:IMSR_JAX:028866 Mouse: Igs7LSL-GCaMP6f (Ai148) The Jackson Laboratory Stock No. 030328; RRID:IMSR_JAX:030328 Mouse: AdvillinFlp Choi et al.62 N/A Mouse: THCreER The Jackson Laboratory Stock No. 025614; RRID:IMSR_JAX:025614 Mouse: Calca-Flp Choi et al.62 N/A Mouse: Bmpr1bCre Sharma et al.13 N/A Mouse: Sstr2CreER Qi et al.43 N/A Mouse: Smr2Cre Qi et al.43 N/A Mouse: Adra2aCreER Qi et al.43 N/A Mouse: TrkBCreER The Jackson Laboratory Stock No. 027214; MGI:5616440 Mouse: CalcaCreER Song et al.63 N/A Mouse: CalcaLox-GFP-STOP-Lox-hDTR (McCoy et al.36 N/A Mouse: Calca-GFP MMRRC Stock No. 011187-UCD Mouse: Mrgpra3-Cre Han et al.64 N/A Mouse: Mrgprb4CreER Vrontou et al.65 N/A Mouse: PIRTCre Patil et al.47 N/A Oligonucleotides Mm-Piezo2 E43-45 ACD Bio Cat#: 439971 Mm-Bmpr1b ACD Bio Cat#: 533941 Mm-Calca TV2-TV3 ACD Bio Cat#: 420361 Mm-Sstr2 ACD Bio Cat#: 437681 Mm-Adra2a ACD Bio Cat#: 425341 Mm-TrkB (NTRK2) ACD Bio Cat#: 423611 MmTH ACD Bio Cat#: 317621 Mm-Trpv1 ACD Bio Cat#: 313338 Hs-Bmpr1b ACD Bio Cat#: 468031 Hs-Calca ACD Bio Cat#: 605551 GCaMP6s ACD Bio Cat#: 557091 tdTomato ACD Bio Cat#: 317041 Software and algorithms ImageJ/FIJI NIH https://ImageJ.nih.gov/ij/; RRID:SCR_003070 GraphPad-PRISM GraphPad N/A JRCLUST Jun et al.66 https://github.com/ JaneliaSciComp/JRCLUST MATLAB Mathworks https://www.mathworks.com/products/ MATLAB.html; RRID:SCR_001622 Python Van Rossum and Drake67 https://www.python.org/ Bonsai Lopes et al.68 https://bonsai-rx.org/ Other Multielectrode arrays Cambridge Neurotech H4-32ch LED probe ThorLabs 470 nm, 17.2 mW (Min) Fiber-Coupled LED Video camera Fisher Scientific Digital USB 2.0 CMOS Ultrasonic microphone Bat Conservation and Management, Inc. Pettersson M500 microphone (Continued on next page) ll OPEN ACCESS Cell 186, 3368–3385.e1–e8, August 3, 2023 e2 Article

    Article Title: Single-Cell Analysis of Regional Differences in Adult V-SVZ Neural Stem Cell Lineages
    Article Snippet: RNAscope Protease III & Protease IV Reagents , ACDBio , Cat No. 322340.

    Next-Generation Sequencing:

    Article Title: Skin-type-dependent development of murine mechanosensory neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit polyclonal anti-S100 Beta Fisher 15146-1-AP chicken polyclonal anti-NFH Aves labs NFH0211 rabbit anti-NF200 Sigma N4142 mouse monoclonal anti-NeuN Millipore AB_2298772 rat polyclonal anti-Troma1 DSHB AB_531826 rabbit anti-DsRed Clontech AB_10013483 goat anti-GFP Abcam AB_305635 goat anti-mCherry Cedarlane AB0040-200 guinea pig anti-vGluT1 Millipore AB_2301751 rabbit anti-Homer1 Synaptic Systems 160003 Isolectin B4 647 Invitrogen SCR_014365 Alexa 546 Life Technologies A11056 Alexa 488, 647 Jackson ImmunoResearch 706-605-148, 703-545-155 Bacterial and virus strains AAV2-retro-hSyn-Cre-WPRE Addgene 105553-AAVrg Chemicals, peptides, and recombinant proteins Tamoxifen Sigma T5648-1g BCIP Sigma 11383221001 NBT Sigma 11383213001 Benzyl Alcohol Sigma 402834 Benzyl Benzoate Sigma B-6630 Sunflower seed oil Sigma S5007 Paraformaldehyde, reagent grade, crystalline Millipore Sigma P6148-500G 10% Neutral buffered saline Sigma HT5012-1CS Critical commercial assays SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara 634894 RNAscope Fluorescent Multiplex Assay ACD Bio 320850 RNAscope Protease III & Protease IV Reagents ACD Bio 322340 RNAscope Probe Diluent ACD Bio 300041 RNAscope Fluorescent Multiplex Assay v.2 ACD Bio 323110 RNAscope H2O2 & Protease Reagents ACD Bio 322381 Opal 570 Akoya FP1488001KT Opal 690 Akoya FP1497001KT Opal 520 Akoya FP1487001KT Experimental models: Organisms/strains Mouse: AvilCre Hasegawa et al.39 and JAX 032536 Mouse: Brn3af(AP) Badea et al.25 and JAX 010558 Mouse: Rosa26FSF-LSL-tdTomato (Ai65) JAX 021875 Mouse: RetCreER Luo et al.19 MGI 4437245 Mouse: TrkBCreER Rutlin et al.17 and JAX 027214 Mouse: AdvillinFlp Choi et al.51 N/A Mouse: TrkBGFP Li et al.22 and JAX 023046 Mouse: Npy2rGFP Li et al.22 MGI 3844094 (Continued on next page) e1 Developmental Cell 58, 2032–2047.e1–e6, October 23, 2023

    Article Title: DRG afferents that mediate physiologic and pathologic mechanosensation from the distal colon.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: R26FSF-LSL-tdTomato (Ai65) The Jackson Laboratory Stock No. 021875; RRID:IMSR_JAX:021875 Mouse: R26LSL-tdTomato (Ai14) The Jackson Laboratory Stock No. 007914; RRID:IMSR_JAX:007914 Mouse: R26FSF-tdTomato (derived from Ai65) The Jackson Laboratory Stock No. 021875; RRID:IMSR_JAX:021875 Mouse: R26FRT-LSL-ChR2-YFP-FRT (Ai32) The Jackson Laboratory Stock No. 012569; RRID:IMSR_JAX:012569 Mouse: R26FSF-LSL-ReaChR The Jackson Laboratory Stock No. 024846; RRID:IMSR_JAX:024846 Mouse: R26FRT-LSL-tdTomato-FRT (Ai9) The Jackson Laboratory Stock No. 007909; RRID:IMSR_JAX:007909 Mouse: R26LSL-GCaMP6s (Ai96) The Jackson Laboratory Stock No. 028866; RRID:IMSR_JAX:028866 Mouse: Igs7LSL-GCaMP6f (Ai148) The Jackson Laboratory Stock No. 030328; RRID:IMSR_JAX:030328 Mouse: AdvillinFlp Choi et al.62 N/A Mouse: THCreER The Jackson Laboratory Stock No. 025614; RRID:IMSR_JAX:025614 Mouse: Calca-Flp Choi et al.62 N/A Mouse: Bmpr1bCre Sharma et al.13 N/A Mouse: Sstr2CreER Qi et al.43 N/A Mouse: Smr2Cre Qi et al.43 N/A Mouse: Adra2aCreER Qi et al.43 N/A Mouse: TrkBCreER The Jackson Laboratory Stock No. 027214; MGI:5616440 Mouse: CalcaCreER Song et al.63 N/A Mouse: CalcaLox-GFP-STOP-Lox-hDTR (McCoy et al.36 N/A Mouse: Calca-GFP MMRRC Stock No. 011187-UCD Mouse: Mrgpra3-Cre Han et al.64 N/A Mouse: Mrgprb4CreER Vrontou et al.65 N/A Mouse: PIRTCre Patil et al.47 N/A Oligonucleotides Mm-Piezo2 E43-45 ACD Bio Cat#: 439971 Mm-Bmpr1b ACD Bio Cat#: 533941 Mm-Calca TV2-TV3 ACD Bio Cat#: 420361 Mm-Sstr2 ACD Bio Cat#: 437681 Mm-Adra2a ACD Bio Cat#: 425341 Mm-TrkB (NTRK2) ACD Bio Cat#: 423611 MmTH ACD Bio Cat#: 317621 Mm-Trpv1 ACD Bio Cat#: 313338 Hs-Bmpr1b ACD Bio Cat#: 468031 Hs-Calca ACD Bio Cat#: 605551 GCaMP6s ACD Bio Cat#: 557091 tdTomato ACD Bio Cat#: 317041 Software and algorithms ImageJ/FIJI NIH https://ImageJ.nih.gov/ij/; RRID:SCR_003070 GraphPad-PRISM GraphPad N/A JRCLUST Jun et al.66 https://github.com/ JaneliaSciComp/JRCLUST MATLAB Mathworks https://www.mathworks.com/products/ MATLAB.html; RRID:SCR_001622 Python Van Rossum and Drake67 https://www.python.org/ Bonsai Lopes et al.68 https://bonsai-rx.org/ Other Multielectrode arrays Cambridge Neurotech H4-32ch LED probe ThorLabs 470 nm, 17.2 mW (Min) Fiber-Coupled LED Video camera Fisher Scientific Digital USB 2.0 CMOS Ultrasonic microphone Bat Conservation and Management, Inc. Pettersson M500 microphone (Continued on next page) ll OPEN ACCESS Cell 186, 3368–3385.e1–e8, August 3, 2023 e2 Article

    Article Title: Single-Cell Analysis of Regional Differences in Adult V-SVZ Neural Stem Cell Lineages
    Article Snippet: RNAscope Protease III & Protease IV Reagents , ACDBio , Cat No. 322340.

    Expressing:

    Article Title: Skin-type-dependent development of murine mechanosensory neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit polyclonal anti-S100 Beta Fisher 15146-1-AP chicken polyclonal anti-NFH Aves labs NFH0211 rabbit anti-NF200 Sigma N4142 mouse monoclonal anti-NeuN Millipore AB_2298772 rat polyclonal anti-Troma1 DSHB AB_531826 rabbit anti-DsRed Clontech AB_10013483 goat anti-GFP Abcam AB_305635 goat anti-mCherry Cedarlane AB0040-200 guinea pig anti-vGluT1 Millipore AB_2301751 rabbit anti-Homer1 Synaptic Systems 160003 Isolectin B4 647 Invitrogen SCR_014365 Alexa 546 Life Technologies A11056 Alexa 488, 647 Jackson ImmunoResearch 706-605-148, 703-545-155 Bacterial and virus strains AAV2-retro-hSyn-Cre-WPRE Addgene 105553-AAVrg Chemicals, peptides, and recombinant proteins Tamoxifen Sigma T5648-1g BCIP Sigma 11383221001 NBT Sigma 11383213001 Benzyl Alcohol Sigma 402834 Benzyl Benzoate Sigma B-6630 Sunflower seed oil Sigma S5007 Paraformaldehyde, reagent grade, crystalline Millipore Sigma P6148-500G 10% Neutral buffered saline Sigma HT5012-1CS Critical commercial assays SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara 634894 RNAscope Fluorescent Multiplex Assay ACD Bio 320850 RNAscope Protease III & Protease IV Reagents ACD Bio 322340 RNAscope Probe Diluent ACD Bio 300041 RNAscope Fluorescent Multiplex Assay v.2 ACD Bio 323110 RNAscope H2O2 & Protease Reagents ACD Bio 322381 Opal 570 Akoya FP1488001KT Opal 690 Akoya FP1497001KT Opal 520 Akoya FP1487001KT Experimental models: Organisms/strains Mouse: AvilCre Hasegawa et al.39 and JAX 032536 Mouse: Brn3af(AP) Badea et al.25 and JAX 010558 Mouse: Rosa26FSF-LSL-tdTomato (Ai65) JAX 021875 Mouse: RetCreER Luo et al.19 MGI 4437245 Mouse: TrkBCreER Rutlin et al.17 and JAX 027214 Mouse: AdvillinFlp Choi et al.51 N/A Mouse: TrkBGFP Li et al.22 and JAX 023046 Mouse: Npy2rGFP Li et al.22 MGI 3844094 (Continued on next page) e1 Developmental Cell 58, 2032–2047.e1–e6, October 23, 2023

    Article Title: DRG afferents that mediate physiologic and pathologic mechanosensation from the distal colon.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: R26FSF-LSL-tdTomato (Ai65) The Jackson Laboratory Stock No. 021875; RRID:IMSR_JAX:021875 Mouse: R26LSL-tdTomato (Ai14) The Jackson Laboratory Stock No. 007914; RRID:IMSR_JAX:007914 Mouse: R26FSF-tdTomato (derived from Ai65) The Jackson Laboratory Stock No. 021875; RRID:IMSR_JAX:021875 Mouse: R26FRT-LSL-ChR2-YFP-FRT (Ai32) The Jackson Laboratory Stock No. 012569; RRID:IMSR_JAX:012569 Mouse: R26FSF-LSL-ReaChR The Jackson Laboratory Stock No. 024846; RRID:IMSR_JAX:024846 Mouse: R26FRT-LSL-tdTomato-FRT (Ai9) The Jackson Laboratory Stock No. 007909; RRID:IMSR_JAX:007909 Mouse: R26LSL-GCaMP6s (Ai96) The Jackson Laboratory Stock No. 028866; RRID:IMSR_JAX:028866 Mouse: Igs7LSL-GCaMP6f (Ai148) The Jackson Laboratory Stock No. 030328; RRID:IMSR_JAX:030328 Mouse: AdvillinFlp Choi et al.62 N/A Mouse: THCreER The Jackson Laboratory Stock No. 025614; RRID:IMSR_JAX:025614 Mouse: Calca-Flp Choi et al.62 N/A Mouse: Bmpr1bCre Sharma et al.13 N/A Mouse: Sstr2CreER Qi et al.43 N/A Mouse: Smr2Cre Qi et al.43 N/A Mouse: Adra2aCreER Qi et al.43 N/A Mouse: TrkBCreER The Jackson Laboratory Stock No. 027214; MGI:5616440 Mouse: CalcaCreER Song et al.63 N/A Mouse: CalcaLox-GFP-STOP-Lox-hDTR (McCoy et al.36 N/A Mouse: Calca-GFP MMRRC Stock No. 011187-UCD Mouse: Mrgpra3-Cre Han et al.64 N/A Mouse: Mrgprb4CreER Vrontou et al.65 N/A Mouse: PIRTCre Patil et al.47 N/A Oligonucleotides Mm-Piezo2 E43-45 ACD Bio Cat#: 439971 Mm-Bmpr1b ACD Bio Cat#: 533941 Mm-Calca TV2-TV3 ACD Bio Cat#: 420361 Mm-Sstr2 ACD Bio Cat#: 437681 Mm-Adra2a ACD Bio Cat#: 425341 Mm-TrkB (NTRK2) ACD Bio Cat#: 423611 MmTH ACD Bio Cat#: 317621 Mm-Trpv1 ACD Bio Cat#: 313338 Hs-Bmpr1b ACD Bio Cat#: 468031 Hs-Calca ACD Bio Cat#: 605551 GCaMP6s ACD Bio Cat#: 557091 tdTomato ACD Bio Cat#: 317041 Software and algorithms ImageJ/FIJI NIH https://ImageJ.nih.gov/ij/; RRID:SCR_003070 GraphPad-PRISM GraphPad N/A JRCLUST Jun et al.66 https://github.com/ JaneliaSciComp/JRCLUST MATLAB Mathworks https://www.mathworks.com/products/ MATLAB.html; RRID:SCR_001622 Python Van Rossum and Drake67 https://www.python.org/ Bonsai Lopes et al.68 https://bonsai-rx.org/ Other Multielectrode arrays Cambridge Neurotech H4-32ch LED probe ThorLabs 470 nm, 17.2 mW (Min) Fiber-Coupled LED Video camera Fisher Scientific Digital USB 2.0 CMOS Ultrasonic microphone Bat Conservation and Management, Inc. Pettersson M500 microphone (Continued on next page) ll OPEN ACCESS Cell 186, 3368–3385.e1–e8, August 3, 2023 e2 Article

    Article Title: Single-Cell Analysis of Regional Differences in Adult V-SVZ Neural Stem Cell Lineages
    Article Snippet: RNAscope Protease III & Protease IV Reagents , ACDBio , Cat No. 322340.



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    https://www.bioz.com/product/rnascope+protease+iii+%26+protease+iv+reagents/rnascope+2+5+ls+protease+iii+digestion/bio_rxiv__2024__02__29__582878-326-19-25
    Average 90 stars, based on 1 article reviews
    rnascope 2.5 ls protease iii digestion - by Bioz Stars, 2026-09
    90/100 stars
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